FIG 2.

Mapping the major 5′ end of sph2 by 5′ RACE. (A) The 5′ ends of the sph2 transcripts from L. interrogans LC82-25 grown in EMJH medium (top) and L. interrogans Fiocruz L1-130 grown in EMJH medium with 80 mM sodium chloride (bottom) were mapped by 5′ RACE. Electropherograms from sequencing reactions of the amplicons are shown. The peaks representing the major 5′ ends of the sph2 transcripts are bisected by a vertical line. The sequence complementary to the start codon is boxed. The direction of transcription is indicated by the long arrow. (B) The nucleotide sequence around the major 5′ end of sph2 is shown. The start codon is shown in bold, and the predicted −10 and −35 promoter elements are underlined.