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. 2018 Nov 15;84(23):e02068-18. doi: 10.1128/AEM.02068-18

FIG 4.

FIG 4

Effect of a transcriptional terminator on background gfp expression. Fluorescence measurements (arbitrary units) of L. interrogans containing plasmids with and without the E. coli rrnD transcription terminator upstream of gfp are shown. The level of GFP produced from a lipL41p-gfp fusion with an upstream rrnD transcriptional terminator is shown for comparison. The dashed line indicates the mean background reading obtained with L. interrogans carrying the empty gfp fusion vector pRAT724. Means ± standard deviations are plotted (n = 4). P values were calculated by one-way analysis of variance (ANOVA) with Tukey's honestly significant difference (HSD) post hoc test.