FIG 5.

GFP production (arbitrary units) from sph2p-gfp fusions in L. interrogans and L. biflexa. Fusion plasmids were introduced into L. interrogans Lai strain 56601 (A), L. interrogans Pomona strain LC82-25 (B), and L. biflexa strain Patoc I (C) and incubated in EMJH medium with spectinomycin. The dashed lines indicate the mean background readings obtained with transconjugants carrying the promoterless gfp fusion vector pRAT724 (none). *, P < 0.05; **, P < 0.01; NS, not significant by Welch's t tests.