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GFP production (arbitrary units) from sph2p-gfp fusions in L. interrogans at different cell densities. Three cultures of each of the transconjugants harboring the fusion plasmids were sampled daily starting from a cell density of 1 × 107 cells/ml. The cells were collected by centrifugation and resuspended in PBS to an OD420 of 0.4 prior to fluorescence measurement. Means ± standard deviations (n = 3) of cell counts (horizontal) and fluorescence readings (vertical) are shown.
