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Regulation of sph2p-gfp fusion expression by sodium chloride. Fusion plasmids were introduced into L. interrogans Lai strain 56601. Fluorescence (arbitrary units) was measured from transconjugants maintained in EMJH medium (EMJH) or EMJH medium with 120 mM sodium chloride (NaCl) for 4 h. Means ± standard deviations are plotted (n = 3). *, P < 0.05; ****, P < 0.0001; NS, not significant by Welch's t tests.
