TABLE 1.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pMaORI | Replicative plasmid for L. interrogans | 24 |
| pGreenTIR | gfp vector | 27 |
| pBluescript SK− | Cloning vector | Stratagene |
| pRAT720 | pMaORI with multicloning site | This study |
| pRAT722 | pGreenTIR with T7 early transcription terminator downstream of gfp | This study |
| pRAT723 | gfp vector in pMaORI, no rrnD transcription terminator | This study |
| pRAT724 | gfp vector in pMaORI | This study |
| pRAT725 | lipL41p-gfp in pRAT724 | This study |
| pRAT727 | sph2pLai-gfp in pRAT724 | This study |
| pRAT728 | sph2pPom-gfp in pRAT724 | This study |
| pRAT729 | sph2pPomΔIS-gfp in pRAT724 | This study |
| pRAT735 | sph2pLai in pBluescript SK− | This study |
| pRAT736 | sph2pPom in pBluescript SK− | This study |
| pRAT739 | sph2pPom(+17)-gfp in pRAT724 | This study |
| pRAT740 | sph2pLai(Δ17)-gfp in pRAT724 | This study |