TABLE 3.
Oligonucleotides used for site-directed mutagenesis
| Oligonucleotide | Sequence (5′→3′)a |
|---|---|
| lip0980p(−206i)-1F | TATTTAAAATAACATTCAATTTAAATAATATTATAATAAAATAAAATTTTATAAAAAATACA |
| lip0980p(−206i)-1R | AATTGAATGTTATTTTAAATAATTTTAGAGAAAGCGGATGATAG |
| la1029p(−222/−206d)-1F | CCTCAAATTA|TTTAAAGAATATTATAATAAAATAAAATTTTATAAAAAATACA |
| la1029p(−222/−206d)-1R | ATTCTTTAAA|TAATTTGAGGACAAGTGGATG |
| la1029p(−222/−206s)-2F | CCTCAAATTATGATTTTTTAGAATAATTTTAAAGAATATTATAATAAAATAAAATTTTATAAAAAATACA |
| la1029p(−222/−206s)-2R | ATTCTTTAAAATTATTCTAAAAAATCATAATTTGAGGACAAGTGGATG |
a
Inserted nucleotides are underlined; deletions introduced at vertical lines; scrambled sequences are in bold font.