Propagation rate of fungivorous nematodes on different A. gossypii transformants. (A) A. avenae (100) or B. okinawaensis (300) nematodes were propagated on A. gossypii VC. Nematodes were harvested and counted after the indicated times of incubation. (B) Indicated FBDPs were individually expressed in the vegetative mycelium of A. gossypii.
A. avenae (100) nematodes were inoculated on individual A. gossypii transformants and incubated for 28 days at 20°C. Thereafter, nematodes were harvested and counted. (C) Indicated FBDPs were individually expressed in the vegetative mycelium of A. gossypii. A total of 300 B. okinawaensis nematodes were inoculated on individual A. gossypii transformants and incubated for 28 days at 20°C. After this period, nematodes were harvested and counted. Each error bar represents the standard deviation of five biological replicates. Dunnett's multiple-comparison test was used for statistical analysis. ns, not significant; *, P < 0.05; **, P < 0.01, ***, P < 0.001; ****, P < 0.0001. Significance was determined versus VC.