. Author manuscript; available in PMC: 2019 Sep 12.

Published in final edited form as: Chem Rev. 2018 Aug 24;118(17):8359–8413. doi: 10.1021/acs.chemrev.8b00238

Table 1.

Substrate Specificity of Endoglycosidases for Hydrolysis of N-Glycans and Glycoproteins

Enzyme	Organisms	GH family	N-Glycan substrate	Core-fucose hydrolyzed?	Assistant residue	General acid/base	ref
Endo-A	Arthrobacter protophormiae	85	HM/Hyb^a	No	N171^b	E173^b	197–199
Endo-M	Mucor hiemalis	85	HM/Hyb/CT^a	No	N175^b	E177^b	200
Endo-D	Streptococcus pneumoniae	85	truncated HM	Yes	N322	E324	201, 202
Endo-CE	Caenorhabditis elegans	85	HM/Hyb^a	No	N152	E154	186
Endo-BH	Bacillus halodurans	85	HM/Hyb^a	ND^c	N170	E172	192
Endo-Om	Ogatea minuta	85	HM/Hyb/CT^a	No	N194	E196	203, 204
Endo-CC1	Coprinopsis cinerea	85	HM/Hyb/CT^a	ND^c	N180	E182	180, 196
Endo-CC2	Coprinopsis cinerea	85	HM/Hyb/CT^a	ND^c	N186	E188	196
Endo-H	Streptomyces plicatus	18	HM/Hyb^a	Yes	D172	E174	205
Endo-F1	Elizabethkingia meningoseptica	18	HM/Hyb^a	Yes	D180	E182	206
Endo-F2	Elizabethkingia meningoseptica	18	CT^a	Yes	D169	E171	194, 206
Endo-F3	Elizabethkingia meningoseptica	18	CT/TCT^a	Preferred	D165	E167	194, 206
Endo-BI1	Bif idobacterium longum subsp. infantis	18	HM/Hyb/CT^a	Yes	D184	E186	207
Endo-BI2	Bif idobacterium longum subsp. infantis	18	HM/CT	ND^c	ND^c	ND^c	207
Endo-S	Streptococcus pyogenes	18	CT	Yes	D233	E235	195, 208–211
Endo-S2	Streptococcus pyogenes	18	HM/Hyb/CT	Yes	D184	E186	210, 212

The abbreviations for N-glycans: HM, high-mannose type; Hyb, hybrid type; CT, biantennary complex type; TCT, triantennary complex type.

The numbering of amino acids refers to the peptide sequence after cleavage of an N-terminal signal peptide as described in the original publications.

Not determined.