Figure 1.

Cultured primary hepatocytes across species were treated with vehicle, UTTR1147A, or positive control. Representative Western blot from 3 separate independent experiments is shown here. Top panel: Concentration‐ and time‐dependent elevations in STAT3 phosphorylation were observed in hepatocytes from human, cynomolgus monkey, minipig, and mouse upon treatment with 0.5 and 5 μg/mL UTTR1147A (0.1 μg/mL rhIL‐22 was used as a positive control) for 5 or 30 minutes. Bottom panel: Higher concentrations (up to 50 μg/mL) and longer duration treatment (up to 24 hours) with UTTR1147A was needed to demonstrate STAT3 phosphorylation in rat hepatocytes. (0.1 μg/mL rhIL‐6 was used as a positive control). Quantification of these blots is presented in Figure A2. STAT3, Signal transducer and activator of transcription 3; pSTAT3, phosphorylated STAT3; rh, recombinant human protein