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. 2018 Nov 16;11:68. doi: 10.1186/s13072-018-0237-6

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Comparison of different methods for chromatin fiber preparation. a Scatter plot depicting the size of CENP-A domain on chromatin fibers prepared with three different methods (left panel). Each dot represents one fiber. Error bars represent mean with SD. n = 32, 28 and 37 fibers for TEEN buffer, single lysis step and double lysis , respectively, from one experiment. Representative pictures of fibers (right panel). Scale bar: 2.5 μm. b Representative pictures of chromatin fibers prepared with three different methods and stained for CENP-A and either CENP-B or CENP-C. Scale bar: 2.5 μm