Fig. 7.

CENP-B and CENP-I are found on the same side when they both spread. a Distribution of TEEN fibers showing spreading outside the centromere domain. Mean with SEM. Dark gray: fibers where only CENP-B spreads, light gray: fibers where only CENP-I spreads and black: fibers where both CENP-B and CENP-I spread. b Distribution of fibers where both CENP-B and CENP-I spread outside the centromere domain. Percentage is the mean of three experiments. A total of 182 fibers were analyzed. c Image of a fiber stained for CENP-C, CENP-B and CENP-I, showing that CENP-B and CENP-I spread on the same side of the centromere domain. Scale bar: 2.5 μm. Intensity plots for each staining along the length of the fiber (in μm) are shown on the right