Fig. 3.

OI inhibits H₂O₂-induced oxidative stress in neuronal cells. SH-SY5Y cells (a-d) or the primary murine neurons (e-f) were pretreated for 30 min with 4-octyl itaconate (OI, 25 μM), followed by stimulation of H₂O₂ (300 μM) for indicated time, relative DCF fluorescent intensity (a and e) and relative superoxide level (b and f) were tested; The lipid peroxidation (c) and DNA damage (d) were tested by TBAR activity assay and p-H2AX FACS assay, respectively. Bars stand for mean ± standard deviation (S.D., n = 5). “Vehicle” stands for PBS control (Same for all Figures). * P < 0.05 vs. “Ctrl” cells. ^# P < 0.05 vs. H₂O₂ treatment only