(A) Clinical scores of EAE in C57BL/6 WT (n = 10) and HDAC11 KO (n = 10) mice immunized with MOG35–55. Results are presented as the mean clinical score ± SEM in each genotype during the course of each experiment. (B) Lesion load determined by area of spinal cord demyelination was assessed by LFB staining. Representative histological sections from 0, 14, 27, and 39 d post-immunization are shown, and the area of demyelination of the spinal cord is delineated by dots. Scale bar, 400 μm. (C) Quantitative comparison of relative lesion load (demyelination) between WT and HDAC11 KO mice. (D) Representative images of Toluidine blue stained spinal cord lesions, 18 and 36 d post-immunization. Scale bar, 100 μm. (E) Quantitative comparison of relative number of preserved axons within the lesion area between WT and HDAC11 KO mice. Data shown are mean ± SD from more than three samples per time point, and P-values were calculated by t test, *P < 0.05.