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. 2018 Sep 24;1(5):e201800039. doi: 10.26508/lsa.201800039

Figure 5. CCL2 expression and migration of macrophages from WT and HDAC11 KO mice.

Figure 5.

(A) Equal numbers of thioglycollate-elicited murine PEMs were isolated from WT (n = 3) and HDAC11 KO (n = 3) mice and cultured in the presence of 1 μg/ml of LPS for 24 h. PEMs were harvested and mRNA was isolated for analysis by qPCR using the CCL2 primers listed in Table S1. (B) PEM culture supernatants were analyzed for CCL2 secretion by ELISA. (C, D) For cell migration assays, PEMs were isolated from WT mice and cultured in Transwell membrane inserts with medium conditioned for 48 h by either WT or HDAC11 KO splenocytes cultured with MOG peptide. The splenocytes were isolated from WT and HDAC11 KO mice with EAE. The number of PEMs migrating across the membranes at different times was counted as an average of three culture wells for each experimental group. Data shown are mean ± SD of three independent experiments, and P-values were calculated by t test, *P < 0.05.