Figure 4. Effects on the assembly of mature respiratory chain complexes by the deletion of SHMT2.

(A–C) BNGE to detect individual mature respiratory complexes in the mitochondrial preparation from the WT and ΔSHMT2 293A cells, followed by visualization with Coomassie blue staining (A), Western blotting with the antibody against a Complex I (CI) marker NDUFS3 (B), or Complex I in-gel activity assay (C). CI, Complex I; CIII₂/IV, CIII₂/IV supercomplex; HSP60, heat shock protein 60.