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. 2018 May 21;1(2):e201800036. doi: 10.26508/lsa.201800036

Figure 5. Effects of glycine and formate on the assembly of Complex I.

Figure 5.

(A, B) Time- and dose-dependent effects on the levels of Complex I core subunit NDUFS1 by the supplementation of the ΔSHMT2 293A cells with glycine (A) or formate (B). Densitometry quantification of NDUFS1 followed by normalization to β-actin is plotted on the top. Data are presented as mean ± SD for three independent experiments. (C, D) BNGE resolution of the mitochondrial preparation from the WT and ΔSHMT2 293A cells that were treated with or without 2 mM formate for 72 h, followed by visualization of the individual mature respiratory chain complexes by the Western blotting probed with the Total OXPHOS antibody cocktail (C) and the Complex I (CI) in-gel activity assay (D). CI, Complex I. (E) Proliferation rates of the WT and ΔSHMT2 293A cells in the galactose media supplemented with or without 2 mM formate. Data are presented as mean ± SD (n = 4).