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. 2018 Feb 15;1(1):e201800026. doi: 10.26508/lsa.201800026

Figure 3. Validation of VCAM1 and DNER as BACE2 substrates in primary mixed glia cultures.

Figure 3.

(A) VCAM1 in medium and cell lysates of Bace1−/− glia treated with vehicle or CpJ (10 μM) for 24 h (n = 4). (B) Quantification of VCAM1 shedding into conditioned medium of Bace1−/− glia treated with vehicle or CpJ for 24 h (paired t test, P = 0.01, n = 4). (C) Quantification of VCAM1 accumulation in cell lysates of Bace1−/− glia treated with vehicle or CpJ (10 μM) for 24 h (paired t test, P = 0.08, n = 4). (D) VCAM1 shedding in WT, Bace1−/−, and Bace2−/− glia (n = 3). (E) VCAM1 in medium and cell lysates of WT glia treated with vehicle or CpJ (10 μM) for 24 h (n = 3). (F) VCAM1 in medium and cell lysates of Bace2−/− glia treated with vehicle or CpJ (10 μM) for 24 h (n = 3). (G) DNER in medium and cell lysates of Bace1−/− glia treated with vehicle or CpJ (10 μM) for 24 h (n = 3). (H) Quantification of DNER shedding into conditioned medium of Bace1−/− glia treated with vehicle or CpJ for 24 h (paired t test, P = 0.03, n = 3). (I) Quantification of DNER accumulation in cell lysates of Bace1−/− glia treated with vehicle or CpJ for 24 h (paired t test, P = 0.3, n = 3). (J) DNER shedding in WT, Bace1−/−, and Bace2−/− glia (n = 3). (K) DNER in medium and cell lysates of WT glia treated with vehicle or CpJ (10 μM) for 24 h (n = 3). (L) DNER in medium and cell lysates of Bace2−/− glia treated with vehicle or CpJ (10 μM) for 24 h (n = 3).