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. 2018 Feb 15;1(1):e201800026. doi: 10.26508/lsa.201800026

Figure 4. Validation of FGFR1 and PLXDC2 as BACE2 substrates in COS-1 overexpression system.

Figure 4.

(A) FGFR1 in lysates and medium of COS-1 cells overexpressing FGFR1 alone (lane 2), FGFR1 with BACE1 (lanes 3 and 4), and FGFR1 with BACE2 (lanes 5 and 6). Lanes 4 and 6 were treated with inhibitor CpJ (10 μM). Lane 1 was mock-transfected with an empty vector. Representative for three experiments. (B) PLXDC2 in medium (top panel) and lysates (second panel from the top) of COS-1 cells overexpressing PLXDC2 alone (lane 2), PLXDC2 with BACE1 (lanes 3 and 4), and PLXDC2 with BACE2 (lanes 5 and 6). Lanes 4 and 6 were treated with inhibitor CpJ (10 μM). Lane 1 was mock-transfected with an empty vector. Control blots for BACE1, BACE2, and actin are shown for each transfection. Representative for three experiments.