(A) Shed VCAM1 and DNER in triplicates of CSF of 11-mo-old WT, Bace1−/−, Bace2−/−, and dKO male mice, as compared with a known BACE1 substrate—SEZ6. (B) SEZ6 is significantly reduced in Bace1−/− and dKO, but not Bace2−/− CSF compared with control WT CSF (one-way ANOVA: P = 0.006, P = 0.008, and P = 1.00, respectively; n = 3). (C) One-way ANOVA revealed no significant differences between shed DNER in Bace1−/−, Bace2−/−, and dKO CSF, as compared with WT (P = 1.00, P = 1.00, and P = 0.77, respectively; n = 3). (D) No significant differences were seen between shed VCAM1 in Bace1−/−, Bace2−/−, dKO, and WT CSF by one-way ANOVA (P = 0.76, P = 1.00, and P = 1.00, respectively; n = 3).