(A) Cytoplasmic localization of YTHDF1, YTHDF2, and YTHDF3 in control, unstressed cells and by silenced “writers” (METTL3, METTL14, and WTAP). The “readers” were detected with Alexa568-conjugated secondary antibody (red). Scale bar, 10 μm. (B) qRT–PCR following siRNA knockdown of YTHDF3 for 48 h in U2OS-G3BP1 cells. The YTHDF3 expression was normalized to the expression of β-actin mRNA. Scr denotes scrambled siRNA and accounts for unspecific effects. (C) mRNA clients of YTHDF1 identified by PAR-CLIP in Wang et al (2015) and compared with the SG transcripts identified in this study. P = 0.006, hypergeometric test. (D) mRNA clients of YTHDF2 identified by PAR-CLIP in Wang et al (2014) and compared with the SG transcripts identified in this study. P = 3.9 × 10−4, hypergeometric test.
Source data are available for this figure.