Table 2.
Changes in expression of genes involved in innate and adaptive immune responses after 3 and 6 h of LPS-induced inflammation
| Gene Name | Fold Change | Gene Functions | |
|---|---|---|---|
| 3 h | 6 h | ||
| Complement component 3 (C3) Rn.11378, NM_016994 |
No change | 7.72 (p = 0.002) |
Activation of the complement system. |
| Chemokine (C-C motif) ligand 12 (Cc112); Rn.137780, NM_001105822 | 8.52 (p = 0.000) |
26.15 (p = 0.035) |
Angiogenesis; cellular response to fibroblast growth factor stimulus; participates in chemokine and cytokine mediated and cytokine signaling pathways. |
| Chemokine (C-C motif) ligand 3 (Cc13) Rn.10139, NM_013025 | 10.62 (p = 0.000) |
14.63 (p = 0.043) |
Monokine with inflammatory and chemokinetic properties. |
| CD 14 molecule (Cd14) Rn.42942, NM_021744 |
7.17 (p = 0.002) |
6.67 (p = 0.002) |
Accessory protein for TLR4. |
| Chemokine (C-X-C motif) ligand 10 (Cxc110); Rn.10584, NM_139089 | 24.23 (p = 0.000) |
NSS | Proinflammatory cytokine; may participate in T-cell effector function and development. Inhibits expression of StAR D1. |
| Intercellular adhesion molecule 1 (1cam1) Rn. 12, NM_012967 | 25.26 (p = 0.000) |
NSS | Up-regulated by TLR2, TLR4, TLR5, and TLR6; ligand for leukocyte adhesion protein. |
| Interleukin 10 (1110) Rn.9868, NM_012854 |
4.43 (p = 0.021) |
No change | Inhibits cytokine synthesis. |
| Interleukin 1 beta (111b) Rn.9869, NM_031512 |
11.32 (p = 0.000) |
14.18 (p = 0.053) |
Endogenous pyrogen, proinflammatory cytokine. |
| Interleukin 6 (116) Rn.9873, NM_012589 |
69.61 (p = 0.000) |
NSS | Disrupts integrity of blood-testis-barrier by altering steady state levels of membrane proteins. |
| Janus kinase 2 (Jak2) Rn.18909, NM_031514 |
No change | 3.77 (p = 0.032) |
Non-receptor tyrosine kinase known to be involved in innate and adaptive immunity signaling. |
| Nuclear factor of kappa light polypeptide gene (Nfkbia) Rn.12550, NM_001105720 |
4.84 (p = 0.000) |
3.99 (p = 0.018) |
Inhibitor of NF-ĸB; binds NF-ĸB and retains it in the cytoplasm. |
| NLF family, pyrin domain containing 3 (Nlrp3) Rn.214177, XM_220513 |
No change | 8.27 (p = 0.001) |
No functional assignments in Uniprot. |
| Solute carrier family 11 (proton-coupled divalent; Slc11a1) Rn.105919, NM_001031658 |
No change | 3.79 (p = 0.000) |
Macrophage protein associated with resistance or susceptibility to intracellular pathogens. |
| Toll-like receptor 1 (Tlr1) Rn. 107,212, NM_001172120 |
No change | 3.36 (p = 0.018) |
Stimulated by bacterial lipoproteins. |
| Toll-like receptor 2 (Tlr2) Rn. 46,387, NM_198769 |
6.35 (p = 0.000) |
8.70 (p = 0.027) |
Increase expression of inflammatory cytokines IL-α, IL-6, and interferon-α, and β |
| Toll-like receptor 5 (Tlr5) Rn. 198,962, NM_001145828 |
−3.37 (p = 0.052) |
No change | Increase expression of inflammatory cytokines IL-α, IL-6, and interferon-α, and β |
| Tumor necrosis factor (TNF superfamily, member 2; Tnf) Rn.2275,NM_012675 |
8.78 (p = 0.000) |
4.58 (p = 0.029) |
Chemokine-mediated signaling, induces germ cell apoptosis in autoimmune orchitis. |
Genes were reported that showed more than a three-fold change following LPS-induced inflammation with p < 0.05. NSS, not statistically significant, greater than three-fold change observed with p > 0.05