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(A) Interaction of FLAG-SYCE2 with HP1α in FLAG-SYCE2–expressing RPE cells unirradiated or irradiated with 8-Gy x-ray 1 h before harvesting the cell lysates. 500 μg of total cell lysates was immunoprecipitated using the anti-FLAG antibody or normal mouse IgG as a negative control and visualized by Western blotting using the anti-HP1α antibody (upper panel).
