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. 2018 Jun 7;1(3):e201800055. doi: 10.26508/lsa.201800055

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© 2018 Berto et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 3. — (A–C) ChIP-qPCR assays to determine the importance of CREB1 and its phosphorylation on S133 for the recruitment of ERα to target genes. MDA-MB-134 cells were transfected and treated as indicated in the figure and in the Materials and Methods section. Note that the fold recruitment values for ERα are experimentally variable between different experiments; these values should only be compared between different treatments and conditions within the same experiment/panel. (D) ChIP-qPCR assays to determine the impact of a siRNA-mediated knockdown of CREB1 on the recruitment of ERα to a wider panel of target genes. CREB1 was knocked down in MDA-MB-134 cells before stimulation as indicated. All values marked with an asterisk are statistically significantly different from their respective vector or siRNA controls with P-values < 0.05.