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. 2018 Jun 7;1(3):e201800055. doi: 10.26508/lsa.201800055

Figure 7. CREB1 and ERα differentially protect breast cancer cells and patients.

Figure 7.

(A) Both CREB1 and ERα suppress STS-induced apoptosis in response to E2 and cAMP. Knockdown of CREB1 and ERα, treatments with STS, E2, and FI, and microscopic assessment of apoptosis were performed with MDA-MB-134 cells as described in the Materials and Methods section. The data points are averages of three independent experiments obtained by the inspection of 200 cells each, and the error bars indicate the standard error of the mean. (B) High expression levels of the CREB1 gene correlate with better breast cancer outcome. The CREB1 gene was used as a marker for the Kaplan–Meier analysis of breast cancers with the GOBO tool. The plots indicate distant metastasis-free survival (DMSF) as a function of time for patients with ERα-positive (ER+) (upper panel) and ERα-negative (ER−) (lower panel) breast cancers. Within each panel, the number of samples in each category and the P-value for the difference between high and low expression cases are shown. Additional analyses are presented in Figs S11 and S12.