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. 2018 Jun 7;1(3):e201800055. doi: 10.26508/lsa.201800055

Figure S3. CREB1 works through the ERα HBD.

Figure S3.

(A) Schematic representation of ERα domains. (B–E) The ERα HBD functionally collaborates with CREB1. Luciferase reporter assays in HEK293T cells with fusion proteins of the DNA-binding domain of Gal4 with the indicated ERα domains: in panels B–E, the A/B domain containing the activation function AF1, the DNA-binding domain (domain C), the hinge (domain D), and the HBD with activation function AF2 (domains E/F). These were transiently coexpressed with wild-type CREB1, CREB1-S133A, and the dominant-negative CREB1 mutant (A-CREB1). (F) Luciferase reporter assay for Gal4-CREB1 activity in HEK293T cells. All values marked with an asterisk are statistically significantly different from their respective vector controls with P-values < 0.05.