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. 2018 Sep 17;1(5):e201800179. doi: 10.26508/lsa.201800179

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© 2018 Ephrussi et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S1. — (A) Schematic representation of transgenic construct GFP-Vas^WT. (B) Expression patterns of nos-Gal4. Schematic representation of the stages at which nos promoter is active (upper panel, green). Confocal image of an ovariole expressing GFP-Vas^WT (GFP signal, green) under the control of nos-Gal4 (bottom panel). (C) Expression patterns of vas-Gal4. Schematic representation of the stages at which vas promoter is active (upper panel, green). Confocal image of an ovariole expressing GFP-Vas^WT (GFP signal, green) under the control of vas-Gal4 (bottom panel). (D) Morphology of ovaries of WT (w¹¹¹⁸), vas^D1/D1; nos-Gal4>GFP-vas^WT, and vas^D1/D1; vas-Gal4>GFP-vas^WT flies. Scale bar indicates 250 μm. (E) Egg-laying rates of WT (w¹¹¹⁸), vas^D1/D1; nos-Gal4>GFP-vas^WT, and vas^D1/D1; vas-Gal4>GFP-vas^WT flies. Error bars represent the standard deviation among three biological replicates (Table S2). (F) Immunodetection of Vasa in early embryos produced by WT (w¹¹¹⁸), vas^D1/D1; nos-Gal4>GFP-vas^WT, and vas^D1/D1; vas-Gal4>GFP-vas^WT females. Scale bar indicates 100 μm (related to Fig 1).