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. 2018 Sep 17;1(5):e201800179. doi: 10.26508/lsa.201800179

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© 2018 Ephrussi et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S3. — (A) Immunodetection of HeT-A/Gag protein in WT (w¹¹¹⁸), (a) vas^D1/D1; nos-Gal4>GFP-vas^WT (b and c) and vas^D1/D1; vas-Gal4>GFP-vas^WT (d) stage 5 embryos. Selected areas of stage 5 embryos are presented in Fig 3A. Scale bar indicates 100 μm. (B) RT–PCR detection of endogenous mnk mRNA in the ovaries of WT (w¹¹¹⁸) flies, mnk^P6/P6, and vas^D1/D1 mutants, as well as vas^D1/D1, mnk^P6/P6 double mutant flies. 18S rRNA was used as a control. (C) Morphology of vas^D1/D1, mnk^P6/P6 double mutant and ago^t2/t3 single mutant ovaries. Scale bar indicates 250 μm (related to Fig 3).