Figure S6. Motif enrichment and codon usage are not correlated.

(A) Definition of a 3-codon motif within the NNS₁₅ library. (B–D) Plots of the log2 (fold change) in frequency of well-measured three-codon motifs for the NoAb sample against the E. coli usage frequency of the (B) first, (C) second, or (D) third codon of each motif. PP(X) or XP(P) motifs that are enriched because of peptide-dependent pausing are shown in yellow. All other motifs are shown in blue and their enrichment is not correlated with codon usage.