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(A) Coomassie Brilliant Blue staining of affinity-purified recombinant laminin-111, laminin-511, and their EQ mutants. SDS–PAGE was carried out in 4% polyacrylamide gels under reducing conditions. (B) Western blot analyses of purified recombinant laminin-111, laminin-511, and their EQ mutants with mAbs against α1, α5, β1, and γ1 subunits to confirm the authenticity of the purified laminin isoforms.
