Figure S3. Nucleolar interactors co-localize with poly-GR/PR in HEK293 cells.

(A–C) HEK293 cells co-expressing RFP-tagged nucleolar interactors NPM1 and NOP56 with GFP, GFP-(GR)₁₄₉, or (PR)₁₇₅-GFP in single focal planes were analyzed. DAPI was used as nuclear marker and the scale bar depicts 20 μm. RFP was used as negative control. Left columns show GFP signal, middle columns RFP-tagged proteins, and right columns show merge of GFP, RFP-tagged proteins, and nuclear DAPI staining (blue). Note that HEK293 cells show co-localization of NPM1 and NOP56 with poly-GR and poly-PR in nucleoli (white arrows). Red arrow indicates cytoplasmic granules occasionally seen in poly-GR/PR–expressing cells.