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Kinetic parameters for Ero1 complexes and Erv1p.
| Enzyme (1 μM) | Activation rate constants | Halftime of activation (min−1) | kcat (s−1) | KM (μM) | Hill constant | |
|---|---|---|---|---|---|---|
| k1 (min−1) | k2 (min−1) | |||||
| Ero1α–PDI | 1.04 ± 0.04 | 1.12 ± 0.06 | 1.56 ± 0.08 | 0.64 ± 0.04 | 5.0 ± 0.8 | 5.2 ± 0.7 |
| Ero1β–PDI | 1.11 ± 0.03 | n.m. | 1.14 ± 0.05 | 1.00 ± 0.02 | 7.6 ± 1.2 | 3.1 ± 0.5 |
| Erv1p | n.m. | n.m. | n.m. | 0.41 ± 0.03 | 89 ± 18 | 1.0 ± 0.1 |
n.m., not measurable.
