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. 2018 Sep 7;1(5):e201800157. doi: 10.26508/lsa.201800157

Figure 4. MBNL1 exon 7-skipping phenotype can be rescued with the depletion of all MBNL1 isoforms.

Figure 4.

(A) Relative cell number change upon co-transfection of siRNA against MBNL1 (50 nM) and ex7-skipping AON (50 nM) in PC3 cells. Parametric paired t test. Each dot represents an independent biological replicate. (B) Cell count of PC3 cells (blue circles), PC3 cells transfected with Cas9 alone (green squares), and PC3 cells transfected with Cas9 and a sgRNA directed against an MBNL1 coding sequence (orange triangles). The cells were transfected with 75 nM of Scrambled AON and MBNL1 AON and counted 48 h posttransfection. Parametric paired t test.