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. 2018 Jun 21;1(3):e201800096. doi: 10.26508/lsa.201800096

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© 2018 Kumbhar et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S3. — Human UBA1 binds to pADPr chains. Left panel: Experimental scheme. Wild-type and mutated versions of ^MBPUBA1^(571–800) were incubated with pADPr polymers, captured on an amylose resin, washed, and eluted with maltose. Right panel: Eluted ^MBPUBA1^(571–800) proteins (wild type and mutants, as indicated) were resolved by PAGE and revealed via anti-MBP immunoblotting. Eluted pADPr was spotted on a nitrocellulose membrane and revealed using an anti-pADPr antibody.