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. 2018 Sep 24;1(5):e201800139. doi: 10.26508/lsa.201800139

Figure 1. Strong interaction of CHGB and lipid membrane.

Figure 1.

(A) SEC profile for purified CHGB. Red triangles indicate molecular weight markers. (B) Silver-stained SDS–PAGE gel of dimers (U2), trimers (U3), tetramers (U4), and high-order oligomers (Un) in CHGB treated with sulfo-SMCC (middle). (C) First eight eigenimages from MSA of a negative-stain EM dataset show C2 symmetry. (D) A cryoEM map for CHGB dimer at ∼10 Å. (E) Top: SDS–PAGE of fractions from a vesicle floatation assay of CHGB in egg PC vesicles in a three-step Ficoll 400 gradient. The top arrow marks the vesicle floatation from bottom (15%) to top (5%). Bottom: quantified CHGB distribution from SDS–PAGE. The floatation experiments were repeated more than 20 times for different batches of CHGB vesicles.