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. 2018 Jul 17;1(4):e201800121. doi: 10.26508/lsa.201800121

Figure 4. TERRA levels are elevated during transformation.

Figure 4.

(A) Northern blot analysis of total RNA from the HLF-derived cell lines. TERRA was detected using a telomeric DNA probe complementary to the UUAGGG repeats. Ethidium bromide (EtBr) staining is shown as a loading control. Two independent biological replicates are shown. (B) TERRA quantification by RT–qPCR with primers specific for the indicated subtelomeric sequences. Plotted is mean + SD from three biological and two technical replicates. Two-tailed unpaired t test, comparing all derived cell lines with primary HLFs (*P < 0.05; **P < 0.01; ***P < 0.001; bars lacking asterisks are not significant).