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. 2018 Jul 17;1(4):e201800121. doi: 10.26508/lsa.201800121

Figure S7. Cellular transformation is not accompanied by accumulation of R-loops.

Figure S7.

(A) Detection of telomeric R-loops by immunoprecipitation using the S9.6 antibody that recognizes DNA/RNA hybrids. As a negative control, half of each nucleic acid preparation was treated with RNase H before the IP. The precipitated DNA was spotted on a membrane and hybridized either with a specific telomere probe (Telo) or a control Alu repeat probe (Alu). Accumulation of telomeric R-loops upon double knockout (DKO) of DNA methyltransferases DNMT1 and DNMT3b in HCT116 cells served as a positive control. (B) Quantification of the S9.6 DNA/RNA IP in (A). The amounts of precipitated DNA were quantified as compared with the total DNA in the inputs. The background RNAse H–resistant signals were subtracted.