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. 2018 Sep 19;1(5):e201800086. doi: 10.26508/lsa.201800086

Figure 2. TSA induces transcription from CCND1-MS2 alleles under CMV promoter control.

Figure 2.

(A) RNA FISH showing the intensity of duplicated CCND1-MS2 sites of transcription (arrows) in control and TSA-treated cells (7 h). The middle column shows the deconvolved image of this cell. Total mRNAs counted: top, 136; middle, 116; bottom, 196. Scale bar, 5 μm. (B) Single molecule RNA FISH quantification of the number of nascent CCND1-MS2 mRNAs on the active sites of transcription (single and doublets) in control and TSA-treated cells (7 h). Number of alleles quantified (n) is marked in red below each box. (C) Single molecule RNA FISH quantification of the total number of CCND1-MS2 mRNAs in the same in control and TSA-treated cells. In the boxplots, the median is indicated by a line in the box, the box represents the interquartile range, the whiskers represent the maximum and minimum values, and dots represent outliers. Number of cells quantified (n) is marked in red below each box.