Figure 3. TSA induces transcription from CCND1-MS2 alleles under CCND1 promoter control.
(A) Single molecule RNA FISH quantification of the number of nascent CCND1-MS2 mRNAs on the active sites of transcription (single and doublets) in control and TSA-treated cells (7 h). (B) Fold-induction levels of transcriptional activity of single genes and duplicated gene after TSA treatment compared with untreated cells (designated as 1). P single = 0.009 and P duplicated = 1.87549 × 10−5. (C) Single molecule RNA FISH quantification of the total number of CCND1-MS2 mRNAs in the same in control and TSA-treated cells. Number of alleles quantified (n) is marked in red below each box.