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. 2018 Sep 19;1(5):e201800086. doi: 10.26508/lsa.201800086

Figure 5. Live-cell imaging of transcriptional activation following TSA treatment.

Figure 5.

(A) Frames from Video 1 showing the activation of the CCND1-MS2 allele under the control of the CMV promoter during TSA treatment. The cell is expressing MS2-CP-GFP. The plot shows the levels of intensity on the active gene over time. Time 0 is the time of TSA addition to the cells. (B) Frames from Video 2 showing the activation of duplicated CCND1-MS2 alleles (CMV driven) during TSA treatment. (C) Frames from Video 3 showing an active single CCND1-MS2 gene (CMV driven), its transition to a duplicated state, and the subsequent activation of the duplicated CCND1-MS2 alleles during TSA treatment. Scale bar, 5 μm.