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. 2018 Sep 19;1(5):e201800086. doi: 10.26508/lsa.201800086

Figure S2. TSA dose-dependent increase in transcription.

Figure S2.

(A) RNA FISH showing the intensity of single CCND1-MS2 sites of transcription (arrows, cells in G1) in control and TSA-treated cells (7 h). Scale bar, 5 μm. (B) Real-time RT–PCR (qRT–PCR) plots showing the changes in CCND1-MS2 mRNA levels in control and TSA-treated cells, under the regulation of the CMV and CCND1 promoters. Levels are relative to the control. Tubulin and 18S rRNA served as loading controls. Mean ± SD are plotted. (C) Plot showing single molecule RNA FISH quantification of the number of nascent CCND1-MS2 mRNAs on the duplicated active sites of transcription in control and TSA-treated cells (7 h). (D) Fold-induction levels of transcriptional activity of single genes after TSA treatment compared with untreated cells (designated as 1). Number of alleles quantified (n) is marked in red.