Table 3.

Enzyme activities of P. pentosaceus MZF16 determined by API-ZYM kit.

Enzyme	Substrate	P. pentosaceus MZF16
Control	−	−
Alkaline phosphatase	2-naphthyl phosphate	−
Esterase	2-naphthyl butyrate	−
Esterase Lipase	2-naphthyl caprylate	−
Lipase	2-naphthyl myristate	−
Leucine arylamidase	L-leucyl-2-naphthylamide	++++
Valine arylamidase	L-valyl-2-naphthylamide	++++
Cystine arylamidase	L-cystyl-2-naphthylamide	++
Trypsin	N-benzoyl-DL-arginine-2 naphthylamide	−
α-chymotrypsin	N-glutaryl-phenylanine-2-naphthylamide	−
Acid phosphatase	2-naphthyl phosphate	++++
Naphtol-AS-BI- phosphohydrolase	Naphtol-AS-BI-phosphate	++
α-galactosidase	6-Br-2-naphthyl-α-D-galactopyranoside	−
β-galactosidase	2-naphthyl-β-D-galactopyranoside	−
β-glucuronidase	Naphtol-AS-BI-β-D-glucuronide	−
α-glucosidase	2-naphthyl-α-D-glucopyranoside	−
β-glucosidase	6-Br-2-naphthyl-β-D-glucopyranoside	++++
N-acetyl-β- glucosaminidase	1-naphthyl-N-acetyl-β-D-glucosaminide	++++
α-mannosidase	6-Br-2-naphthyl-α-D-mannopyranoside	−
α-fucosidase	2-naphthyl-α-L-fucopyranoside	−

(−), negative (colorless or very pale yellow); (+), positive (violet or orange or blue).