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. 2018 Nov 9;9:2535. doi: 10.3389/fimmu.2018.02535

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Copyright © 2018 Gazzinelli-Guimarães, Gazzinelli-Guimarães, Nogueira, Oliveira, Barbosa, Amorim, Cardoso, Kraemer, Caliari, Akamatsu, Ho, Jones, Weatherhead, Bottazzi, Hotez, Zhan, Bartholomeu, Russo, Bueno and Fujiwara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental immunization with Ascaris suum antigen-induced antibody responses. Antigen-specific antibodies from immunization with extracts of the infective larvae L3 (ExL3), adult worms (ExAD), and the cuticle of adult worms (CUT) were measured by ELISA in BALB/c mice during and after immunization. (A) Ascaris-specific IgG before first immunization (Time 0), post first immunization (Time 1), post second immunization (Time 2), and post three immunizations (Time 3). IgG1 (B), IgG2a (C), IgG2b (D), and IgG3 (E) in the serum of mice after the third immunization (Time 3) with the A. suum antigens. (F) Ascaris-specific IgE. (G) Ascaris-specific IgA. Specific recognition of the proteins As16 (H) and As14 (I) by antibodies of mice immunized with larval antigen (ExL3), crude extract of adult worm (ExAD), and cuticle (CUT). Statistically significant differences (p < 0.05) were represented by ^* compared to the control group MPLA in which ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.