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. 2018 Nov 9;9:2535. doi: 10.3389/fimmu.2018.02535

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Copyright © 2018 Gazzinelli-Guimarães, Gazzinelli-Guimarães, Nogueira, Oliveira, Barbosa, Amorim, Cardoso, Kraemer, Caliari, Akamatsu, Ho, Jones, Weatherhead, Bottazzi, Hotez, Zhan, Bartholomeu, Russo, Bueno and Fujiwara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunization with different A. suum extracts had distinct effects on leukocyte influx into the airways following A. suum infection. Inflammatory cells in the bronchoalveolar lavage (BAL) after immunization with the extracts and challenge with A. suum were quantified using a Neubauer chamber and cytospin preparations. (A) Total leukocytes. (B) Eosinophils. (C) Neutrophils. (D) Macrophages. (E) Lymphocytes. (F) Total protein. Statistically significant differences (p < 0.05) were plotted compared to the control groups PBS-NI and PBS-ASC in which ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.