Fig. 7. Fluorescence quantification of amplified DNA products from RCA reactions using the cyclic templates 1_PO4, 1_Am, 1_PA and 1_Tz and the φ-29 DNA polymerase over a 10 hour time course. EDTA was added to break down the precipitate releasing extended DNA prior to incubation with DNA binding dyes. Measurements are recorded in the presence of SYBR Gold, SYBR Green I and SYBR Green II fluorescent DNA binding dyes. Error bars represent the standard deviation of three measurements taken from a single RCA reaction. The reactions were also repeated in duplicate and the same trends were observed in both cases.
