Table 1. Showing the peptide sequences and spacers incorporated into dual-probe scaffold.
Compound | Sequence 1 (thrombin substrates) | Sequence 2 (MMP substrates) | X2 |
1 | –NleWPRGWRL | –GPKGLKG– | –K(PEG2)-PEG2– |
2 | –NleWPRGWRL– | –PFGNleKβA– | –K(PEG2)-PEG2– |
3 a | –NleWPRGWR(d)L– | –PFGNleKβA– | –K-PEG2– |
4 a | –NleWP(d)RGWR(d)L– | –PFGNleKβA– | –K-PEG2– |
5 a | –NleWPRGWR(d)L– | –PFG(d)NleKβA– | –K-PEG2– |
a d-Aminoacids d-Leu, d-Arg and d-Nle were used where indicated. X1: –PEG2-K(–CO(CH2)2–C CH). Tail: -[PEG2-(d)Lys]3-NH2; Nle:Norleucine.