Left panels: Depiction of TFx and PSA over time for 3 patients in the cohort treated with radiation therapy (RT). Note that only patient 19 had plasma collected for cfDNA on a day when he received external beam radiation therapy. Two markers from the same time point on days 112 and 196 for patient 20, and on day 196 for patient 19 denote replicates from the same blood draw but with independent cfDNA isolation, library construction, and sequencing steps. Right panels: Depiction of total cfDNA yield, tumor DNA yield, and noncancerous (“normal”) DNA yield over time in the same 3 patients.