Multiplex immune profiling of 45 inflammatory cytokines and chemokines using serum specimens derived from 30 healthy pregnant women (n = 10/trimester) and 30 symptomatic ZIKV+ women (n = 10/trimester) from Rio de Janeiro, Brazil (RJ). (A) Heatmap of 45 inflammatory cytokines and chemokines that were significantly increased/decreased when compared with RJ healthy controls. Fold changes in cytokines are relative to RJ healthy controls and presented as heatmap. Red, white, and blue indicate induction, no change, and repression, respectively. (B) Venn diagram representation of number of inflammatory cytokines and chemokines significantly induced/repressed during symptomatic ZIKV+ pregnancy. (C) IPA analysis of proteins predicts the connection of cytokines with various ZIKV-associated clinical manifestations.