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. 2018 Nov 2;3(21):e95076. doi: 10.1172/jci.insight.95076

Figure 2. Expression and colocalization of ALMS1 and NKCC2 in rat TAL.

Figure 2

(A) Representative immunoblot of ALMS1 indicating ALMS1 protein expression in rat thick ascending limb (TAL) corresponding to 230 kDa protein; n = 4. (B) Representative image for immunofluorescent labeling of ALMS1 in isolated, perfused TAL, indicating apical and subapical vesicular localization of ALMS1 by confocal imaging in a cross-section of TAL; n = 3. Scale bars: 5 μm. (C) Representative image for immunofluorescent labeling of NKCC2 and ALMS1 in rat TAL primary cultured cells; n = 3. NKCC2 (green); ALMS1 (red); tight junction protein zonula occludens 1 (blue). Merged image from a subapical plane (1.5 μm from the apical plane) showing overlap of internalized NKCC2 and ALMS1 indicated with an arrow. Scale bars: 5 μm. (D) Representative image for immunofluorescent labeling of NKCC2 (green) and ALMS1 (red) in a single TAL cross-section obtained from perfusion-fixed rat kidney slices (n = 4). Scale bars: 10 µm.