Skip to main content
. 2018 Nov 2;3(21):e95625. doi: 10.1172/jci.insight.95625

Figure 3. Offspring of pregnant rats treated with dexamethasone (Dex).

Figure 3

(A) Levels of Dnmt1 (n = 7) and Dnmt3a (n = 11) mRNA in control and Dex-treated offspring, and levels of Agtr1a and Agtr1b mRNA in control (n = 13 and 9, respectively) and Dex-treated (n = 9 and 6, respectively) offspring. (B) Bisulfite sequence analysis of the Agtr1a locus in the PVN from 12-week-old control and Dex-treated offspring. Top, schematic diagram of the Agtr1a locus. Dashes and numbers indicate the positions of the cytosine residues of CpG dinucleotides relative to the TSS (+1). Bottom, DNA methylation status of the CpG sites between +114 and +214 bp relative to the TSS. Filled circles, methylated CpG sites; open circles, demethylated CpG sites. (C) ChIP assays showing DNMT1 (top) and DNMT3a (bottom) binding to the sites –97 to +107 (n = 6 and 8 for DNMT1, and n = 9 and 11 for DNMT3a, respectively) and +174 to +366 relative to the TSS (n = 4 and 4 in DNMT1, and n = 10 and 8 in DNMT3a, respectively), in the PVN of control and Dex-treated offspring. Filled circles, control; open circles, Dex-treated offspring. Throughout, data represent the means ± SEM. *P < 0.05 versus control offspring (t test).